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. 2015 Dec 9;6:1098. doi: 10.3389/fpls.2015.01098

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Copyright © 2015 Egusa, Matsui, Urakami, Okuda, Ifuku, Nakagami and Kaminaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of chitin nanofiber. (A) Chitin nanofiber consists of long polymeric chitin. The water-soluble fraction from chitin powder suspension (chitin powder) and chitin nanofiber dispersant (CNF) were subjected to HPLC analysis. Chitin-oligosaccharides (GlcNAc)_2-6 (Chitin 2-6 mer) were used as the positive control. (B) Comparison of chitinolytic enzyme sensitivity. Enzymatic degradation of chitin before (Chitin powder) and after nanofibrillation (CNF) was analyzed using a chitinase assay. Similar result obtained in four independent experiments and the data show one representative.