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. 2015 Dec 9;6:1098. doi: 10.3389/fpls.2015.01098

FIGURE 2.

FIGURE 2

Generation of reactive oxygen species (ROS) by chitin nanofiber treatment. ROS production was measured by chemiluminescence mediated by L-012 after elicitor treatment. (A) ROS production in 10-day-old Arabidopsis seedlings. Wild-type Col-8 or cerk1-2 mutant plants was treated with 1 mg/mL or 0.1 mg/mL chitin nanofiber (CNF) or 1 mg/mL chitin-oligosaccharides (GlcNAc)2-6 (Chitin 2–6 mer). (B) ROS production in suspension-cultured rice cells treated with 0.1 mg/mL CNF, chitin-oligosaccharides (GlcNAc)2-6 (Chitin 2–6 mer) or N-acetylchitohexaose (Chitin 6 mer). HRP: horseradish peroxidase. The values represent the average and standard errors of six replicate experiments.