Table 6. Validation of RNA-sequencing results by real-time PCR.

Comparison	Gene	Regulated direction	FC by qRT-PCR	p-value by qRT-PCR	LogFC by RNA-seq	FDR by RNA-seq
WT-GBM vs WT-Nor	Ighg1	Up	161.1160	0.0640	13.2864	6.93E–30
	Igkc	Up	2.2981	0.0020	9.9349	4.04E–32
	Cox6a2	Down	0.3434	0.0456	−5.6157	5.25E–10
	Angptl7	Down	0.2022	0.0043	−5.6949	4.17E–46
	Sftpc	Down	0.0235	0.0246	−11.4023	5.63E–05
WT-UUO vs WT-Nor	Il1rn	Up	105.1993	0.0006	10.5668	7.98E–26
	Trem1	Up	4.0638	0.0185	8.7348	1.19E–18
	Flna	up	6.2702	0.0070	17.2533	1.40E–54
	Pvalb	Down	0.0051	0.0000	−6.9307	2.20E–13
	Ugt1a9	Down	0.4222	0.0001	−6.2263	1.81E–11
	Sftpc	Down	0.0591	0.0270	−11.3729	8.30E–05
KO-GBM vs KO-Nor	Rpl29	Up	1.8738	0.0331	7.0270	9.69E–16
	Ceacam2	Up	6.3943	0.0179	5.4004	1.91E–13
	Igj	Down	0.1877	0.0023	−3.5747	2.70E–05
	Hrg	Down	0.3559	0.0379	−8.21951	4.07E–04
KO-UUO vs KO-Nor	Col11a1	Up	5.5698	0.0071	6.9993	5.48E–07
	Ccl7	Up	23.4986	0.0055	7.228708	4.57E–02
	Cyp3a11	Down	0.4963	0.0165	−7.8656	5.78E–08
	Serpina1a	Down	0.7025	0.0470	−8.8200	2.54E–20

Note: WT-GBM:anti-GBM GN model in wild-type mice; WT-Nor:wild-type normal mice; WT-UUO:UUO model in wild-type mice; KO-GBM:anti-GBM GN model in Smad3 knockout mice; KO-UUO:UUO model in Smad3 knockout mice; KO-Nor:Smad3 knockout normal mice.