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. 2015 Oct 30;4(10):e47. doi: 10.1038/cti.2015.21

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Copyright © 2015 Australasian Society for Immunology Inc.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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HLDA10 mAb binding to both thymic cDC subsets but not to pDC. HLDA10 mAb reacting with cDC but not pDC. Dot plots show stainings as overlays of CD141⁺ (red) versus CD11b⁺ (green) cDC (top row), or pDC (orange) within total thymocytes (dark gray) (bottom row). Inset numbers give the proportion of the respective DC populations stained. Gates were set according to background controls (FMO for fluorophore-labeled mAb, secondary reagent controls for IgM or biotinylated mAb or isotype controls for unlabeled IgG mAb). Histogram overlays (middle rows) show signals of the panel mAb obtained with the color-coded thymic DC populations (open histograms) in comparison with the background staining (filled-in gray). The minimal signals with mAb to CD1a, CD1b, CD1c and CD101 on pDC were regarded as background. The reactivity with other thymic cell populations can be seen in Table 1. Shown are representative data from one out of three thymic tissues.