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. 2015 Nov 27;47(11):e196. doi: 10.1038/emm.2015.87

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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PLD2 directly interacts with HIF-1α. (a) HEK293 cells were transfected with the indicated constructs, and coimmunoprecipitation was performed to examine the interaction of HIF-1α with PLD2 in the presence of MG132 (2.5 mM) for 6 h. (b) Immunoprecipitation assay of in vitro translated-HA-HIF-1α and PLD2. (c) GST pull-down assay of binding domain mapping of PLD2. A schematic representation of the PLD1 domain is shown. The data are representative of three independent experiments.