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. 2015 Oct 16;47(10):e190. doi: 10.1038/emm.2015.72

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Copyright © 2015 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Rac knockdown inhibits the leptin-induced increase in K_ATP channel activity and leptin-induced hyperpolarization. (a) Representative single K_ATP channel currents of siCtrl-, siRac1-, or siRac2-transfected INS-1 cells pretreated with or without 10 nM leptin. The inside-out patches were held at −60 mV. C, closed level. O, opened level. (b) Mean K_ATP channel activity (NPo) of siCtrl-, siRac1-, or siRac2-transfected INS-1 cells pretreated with or without 10 nM leptin (siCtrl/11 G, 0.21±0.06, n=15; siCtrl/11 G+Leptin, 0.83±0.23, n=9; siRac1/11 G, 0.15±0.04, n=11; siRac1/11 G+Leptin, 0.27±0.15, n=12; siRac2/11 G, 0.24±0.12, n=10; siRac2/11 G+Leptin, 0.31±0.1, n=12). Data are represented as the mean±s.e.m. **P<0.01 compared with siCtrl/11 G. (c) Representative traces for the effects of leptin on membrane potentials from siCtrl-, siRac1-, or siRac2-transfected INS-1 cells. (d) Summary data for mean resting membrane potential (RMP) of INS-1 cells transfected with siCtrl, siRac1, or siRac2 before or after the application of leptin (siCtrl/11 G, −37.0±2.6 mV, n=8; siCtrl/11 G+Leptin, −64.2±3.4 mV, n=8; siRac1/11 G, −32.0±3.9 mV, n=7; siRac1/11 G+Leptin, −34.7±3.2 mV, n=7; siRac2/11 G, −29.5±2.6 mV, n=8; siRac2/11 G+Leptin, −29.8±3.0 mV, n=8). Data are represented as the mean±s.e.m.