Figure 6. SFN inhibits the NLRP3 and NAIP5/NLRC4 inflammasomes in an Nrf2-independent manner.

Nrf2^−/− or wild-type C57BL/6J BMDMs were treated with LPS (1 μg/ml) for 2 h, then with or without SFN (50 μM) for 30 min, followed with or without nigericin (A; 50 μM) for 30 or 60 min or FlaTox (B; 1 μg/ml) for 60 or 90 min. Cell lysates were prepared and IL-1β processing was assessed by Western blotting of cell lysates (lys) and supernatants (sup). Cross-reactive is a nonspecific cross-reactive band to demonstrate equal protein loading.