Figure 7. METH increases TAAR1 expression in the presence of HIV-1 infection.
PBMCs were treated with or without METH for 24 h and then infected with HIV-1ADA for 4 h, washed. and cultured in complete RPMI medium with IL-2. PBMCs were harvested on d 6 postinfection, and TAAR1 expression was analyzed by Western blot. (A) TAAR1 was detected as a 39–40 kDa protein. Actin was used as a loading control. Bands were analyzed from the same blot with 1 sample lane rearranged for the figure. (B) The bar graph represents fold change in band intensity from untreated control after normalization with actin. Unactivated control was without any PHA stimulation and without any treatment. The rest of the conditions were PHA activated and then treated with or without METH or HIV-1ADA. The blot is representative of 3 independent testings.