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. 2015 Nov 23;6:8869. doi: 10.1038/ncomms9869

Figure 6. The TATA element potentiates pSER-dependent transcriptional activation.

Figure 6

(a) Schematic structures of the GAL4-responsive reporter with or without the TATA element. The FR–LUC reporter is termed ‘Control' and the TATA-deficient version lacking the TATATA sequence of the TATA element is termed ‘dTATA'. (b) Requirement for the TATA element for transactivation by various ADs. 293T–RL–LUC cells carrying the TK–RL reporter and the FR–LUC reporter, with (control) or without (dTATA) the TATA element, were transiently transfected with the pCMV5 expression vectors for various GAL4 fusion proteins. Promoter activity was assessed with the dual luciferase reporter assay. Transcription activation activities, normalized to the RL activity, are shown relative to the value of each control (arbitrarily set as 100). (c) Localization of FLAG-tagged GAL4–AD fusion proteins, TAF1C and TBP on the chromatinized luciferase promoter with or without the TATA element. Each FLAG-tagged GAL4–AD fusion protein was expressed in 293T–RL–LUC (control or dTATA) cells and subjected to fanChIP–qPCR using the indicated antibodies. Precipitated DNA was analysed with qPCR, using a probe specific to the pre-transcription start site (TSS) region, as shown in a.