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. 2015 Nov 23;6:8869. doi: 10.1038/ncomms9869

Figure 7. The SDE and NKW motifs collaborate to initiate transcription.

Figure 7

(a) Alignment of the amino acid sequences of the SDE and NKW motifs of human AF4 family proteins. Multiple alignments were performed using the Clustal W software. ‘*', 100% amino acid identity; ‘.',75% amino acid similarity. The SDE and NKW motifs are shown in open red boxes. (b) Transforming ability of P′C′+ mutants fused with various pSER subdomains. Hoxa9 expression in the first round colonies and the colony-forming units (CFUs) at the third and fourth rounds of replating are shown as described in Fig. 5a. (c) Protein expression of the P′C′+ mutants fused with various AF4-2C subdomains in the packaging cells. (d) Transactivation by various pSER subdomains. The ability to recruit SL1 is indicated. A transactivation assay was performed as described in Fig. 5c. (e) Association of SL1 components with various pSER subdomains. FLAG-tagged GAL4-pSER subdomains fusion proteins were expressed in 293T–LUC cells and subjected to fanChIP–WB. The sample shown in the input lane is indicated by an asterisk. (f) Localization of FLAG-tagged GAL4-pSER subdomain fusion proteins, TAF1C and TBP on the chromatinized luciferase promoter. Each FLAG-tagged GAL4-pSER subdomain fusion protein was expressed in 293T–LUC cells and subjected to fanChIP–qPCR as described in Fig. 5e.