Table 1.
Summary of analyzed parameters for targeted preamplification.
| Tested parameter | Final primer concentration (nM) | Number of assays | Annealing time (min) | Annealing temperature (°C) | Template concentration |
|---|---|---|---|---|---|
| Template concentration (Figure 2) |
40 | 96 | 3 | 60 | 6 × 100 molecules + 90 × 0–107 molecules |
| Template concentration (Figure 3) | 40 | 96 | 3 | 60 | 95 × 100 molecules + 1 × 100–109 molecules |
| Number of assays (Figure 4) | 40 | 6, 12, 24, 48, 96 |
0.5, 3, 8 | 60 |
96 × 100 molecules |
| Primer concentration (Figure 5) | 10, 40, 160, 240 | 96 | 0.5, 3, 8 | 60 | 96 × 100 molecules |
| Annealing temperature (Figure 6) | 40 | 96 | 3 | 55.0, 56.8, 58.8, 61.3, 63.3, 65.3 | 96 × 100 molecules |
| PCR Additives (Figure 7) |
40 | 96 | 3 | 60 |
96 × 100 molecules |