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. 2015 Dec 9;5:17187. doi: 10.1038/srep17187

Figure 7. Comparison of slice support materials and slice incubation cultivation systems.

Figure 7

(a) H&E stained and HIF1α immuno stained sections of MCF-7 CDX tumour slices cultivated using an adherent filter support with a 5 μm pore size (Alvetex Strata), a 0.4 μm pore size (Alvetex Polaris) and using a tissue slice incubation unit supported by titanium grids, which were periodically rotated into the media and air (see Methods). (b) H&E stained sections of Lung GEMM tumour slices annotated for regions of necrosis in slices cultivated using Millipore filters and a tissue slice incubation unit. Pink shaded areas indicate necrosis. (c–f) PCA plot of the stress gene biomarker expression profiles and corresponding euclidean distance scatterplots of MCF-7 CDX and PC-295 PDX tumour slices cultivated using different support methods. Scale bars represent 100 μm. Key: M  = Millipore Filter, P = Alvetex Polaris Filter, S = Alvetex Strata Filter, IU = Tissue slice incubation unit. Results are from three independent experiments.