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. 2015 May 15;50(10):1241–1252. doi: 10.3109/00365521.2015.1038849

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© 2015 The Author(s). Published by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — Detection of peptides/proteins (IHC) and mRNA (ISH). (A) Immunoreactivity score (IRS) for GN and GC-C in healthy mucosa, un-inflamed IBD patients (IBD-U) and inflamed IBD. Healthy (n = 6) is indicated with target circle, UC (n = 5) and UC-U (n = 5) with filled circles and CD (n = 3) and CD-U (n = 5) with open circles. Mean IRS for GN was 11.33, 7.70 and 3.38 for Healthy, IBD-U and IBD. Mean rank difference for GN was 6.50 for Healthy vs. IBD-U (p = 0.14) and 13.63 for Healthy vs. IBD (p = 0.0006). Mean IRS for GC-C was 8.67, 6.55 and 4.56 for Healthy, IBD-U and IBD. Mean rank difference for GC-C was 5.05 for Healthy vs. IBD-U (p = 0.32) and 9.44 for Healthy vs. IBD (p = 0.02). (B) Representative GN and GC-C stained tissue specimens from Healthy, IBD-U and IBD (Magnification, 200×). (C) Mucosal expression of GUCA2A/Guca2a, GUCA2B/Guca2b and GUCY2C/Gucy2c in endoscopic biopsies from healthy human colon and from endoscopic biopsies from healthy and inflamed rat colon after induction of TNBS colitis. There is marked expression of GUCA2A/Guca2a and quite significant expression of GUCY2C/Gucy2c in healthy colonic mucosa. After induction of TNBS colitis only a few cells are positive for Guca2a (arrows with open arrowheads) and Gucy2c (dashed arrow). The pattern of expression is similar in healthy human and rat colon. GUCA2A/Guca2a and GUCY2C/Gucy2c are expressed in both goblet and columnar cells. Guca2b has no noticeable expression in rat samples neither before nor after colitis induction, whereas in human colonic mucosa GUCA2B is strongly expressed in solitary epithelial cells (arrows with solid arrowheads) and weaker in other epithelial cells (Magnification, 200×).