Table II.
FC with p-Values | ||||
---|---|---|---|---|
Human | UC vs. Healthy | CD vs. Healthy | ||
FC | p | FC | p | |
GUCA2A | −4.91 | 3.28 × 10−13 | −2.86 | 0.0037 |
GUCA2B | −6.06 | 1.01 × 10−16 | −4.64 | 7.12 × 10−6 |
GUCY2C | −1.61 | 1.27 × 10−11 | −1.36 | 0.010 |
TNBS colitis | Day 3 |
Day 7 |
Day 12 |
|||
---|---|---|---|---|---|---|
FC | P | FC | p | FC | p | |
Guca2a | −3.47 | 0.0003 | −2.40 | 0.0006 | −1.79 | 0.0009 |
Guca2b | −1.05 | 0.82 | 2.22 | 0.0032 | 1.78 | 0.034 |
Gucy2c | −2.88 | 0.0002 | −2.36 | 0.0008 | −2.02 | 0.0024 |
Log2 gene expression in Healthy, un-inflamed IBD (IBD-U) and IBD Left colon and IBD Right colon |
Gene | Healthy (n = 20) |
IBD-U (n = 61a) |
IBD Left colon (n = 33) |
IBD Right colon (n = 11) |
pb, f | |||||
---|---|---|---|---|---|---|---|---|---|---|
Log2 expression (SD) | Log2 expression (SD) | pb, c | Log2 expression (SD) | FC vs. Healthy | pb, d | Log2 expression (SD) | FC vs. Healthy | pb, e | ||
GUCA2A | 13.42 (0.21) | 13.32 (0.29) | >0.99 | 11.29 (1.37) | −4.08 | <0.0001 | 11.11 (1.78) | −4.63 | <0.0001 | >0.99 |
GUCA2B | 10.85 (0.33) | 10.90 (0.56) | >0.99 | 8.30 (1.21) | −6.06 | <0.0001 | 8.30 (1.44) | −6.06 | 0.0003 | >0.99 |
GUCY2C | 9.92 (0.20) | 9.92 (0.25) | >0.99 | 9.26 (0.42) | −1.59 | <0.0001 | 9.34 (0.38) | −1.51 | 0.0019 | >0.99 |
Abbreviations: CD = Crohn’s disease; FC = Fold change; IBD = Inflammatory bowel disease; TNBS = 2,4,6-Trinitrobenzene sulphonic acid; UC = Ulcerative colitis.
aDenotes the number of samples from both IBD patients in remission without inflammatory changes, as well as samples from endoscopically and histologically un-inflamed hepatic flexure of IBD patients with inflammatory changes elsewhere in the colon.
bData analyzed with the Kruskal–Wallis test and Dunn’s multiple comparisons test.
cComparison between Healthy and IBD-U.
dComparison between IBD Left colon and Healthy.
eComparison between IBD Right colon and Healthy.
fComparison between IBD Left colon and IBD Right colon.