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. 2015 Mar 19;50(3):231–241. doi: 10.3109/10409238.2015.1023252

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© 2015 The Author(s). Published by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — Single vesicle–vesicle lipid-mixing assays with tethered vesicles. (A) “Acceptor” vesicles containing v-SNAREs (synaptobrevin shown in blue) and labeled with DiD (red phospholipid head groups) and biotin (yellow) are immobilized to a PEG-biotin coated surface through linkage with neutravidin (navy blue). Donor vesicles containing t-SNAREs (syntaxin shown in red and SNAP-25 shown in green) and labeled with DiI (green phospholipids) are added and fusion is measured by measuring FRET using TIR microscopy (Yoon et al., 2006). (B) Synaptotagmin (light purple) was added in the lipid-mixing study by Lee et al. (2010).