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. 2015 Mar 19;50(3):231–241. doi: 10.3109/10409238.2015.1023252

Figure 6.

Figure 6.

Proteoliposome reconstitution schemes. Outlined are three methods to create proteoliposomes with reconstituted purified synaptic proteins and various reporter fluorescent dyes. The modified standard method (Kyoung et al., 2011, 2013) produces vesicles with uniform size and protein distribution. This method has been used for the single vesicle content/lipid-mixing assay described in Figure 5(A–C).