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. 2015 Nov 5;14(12):5291–5305. doi: 10.1021/acs.jproteome.5b00746

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Copyright © 2015 American Chemical Society

This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.

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Structural analysis of core modifications of N-glycans isolated from the fut-1;fut-6 strain. N-glycans (Hex_4–6HexNAc₂Fuc_2–3Me_0–2-PA) separated by either RP-HPLC or 2D-HPLC were subject to various treatments. A 9.4 g.u. RP fraction containing three major glycans (m/z 1457, 1605, and 1781) was incubated with either jack bean α-mannosidase or α-galactosidase (A–C). Two 2D-HPLC fractions containing trifucosylated structures (m/z 1751 and 1779) were treated either with hydrofluoric acid and bovine α-fucosidase (D–F) or sequentially with hydrofluoric acid and Aspergillus β-galactosidase (G–I). Key core MS/MS fragments of relevant N-glycans are shown in the right panels.