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. 2015 Dec 9;5:18007. doi: 10.1038/srep18007

Figure 5. The effect of IL-1R2 and IL-1Ra on IL-1-induced chemokine production.

Figure 5

(a) The effect of IL-1β on the mRNA levels of IL-1 receptors. HMC cells were treated with 20 ng/ml IL-1β for the indicated time periods, the mRNA levels of IL-1R1, and IL-1R2 were detected by RT-PCR. GAPDH mRNA levels were detected as loading controls. (b) The effect of IL-1β on the protein levels of IL-1R1. HMC cells were treated with the indicated concentrations of IL-1β for 24 h. The protein levels of IL-1R1 (80 KD) were detected by western blot. GAPDH (36 KD) protein levels were detected as loading controls. (c) The effect of IL-1Ra on the mRNA levels of CXCL1, 2, and 8 induced by IL-1β. HMC cells, pre-treated with the indicated concentrations of IL-1Ra for 1 h, were re-stimulated with 5 ng/ml IL-1β for 1 h. The mRNA levels were detected by qRT-PCR. (d) The effect of IL-1Ra on the mRNA levels of CXCL1, 2, and 8 induced by IL-1α. (e) The effect of IL-1β on the mRNA levels of IL-1Ra in HMC cells and in human monocytic THP-1 cells. p-value *< 0.05, **< 0.01, and ***< 0.001 compared with the IL-1 treated alone groups.