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. 2015 Dec 9;5:18007. doi: 10.1038/srep18007

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(a) IL-1β dose-dependent regulation of A20 mRNA. HMC cells were treated with indicated concentrations of IL-1β for 1 h. The mRNA levels of A20 were detected by qRT-PCR. (b) IL-1β time-dependent regulation of A20 mRNA. HMC cells were treated with 5 ng/ml IL-1β for the indicated time periods. The mRNA levels of A20 were detected by qRT-PCR. (c) IL-1β time-dependent regulation of A20 (82 KD) protein. (d) IL-1β dose-dependent regulation of A20 protein. (e) IL-1α dose-dependent regulation of A20 mRNA. (f) IL-1α time-dependent regulation of A20 protein. p-value **< 0.01, and ***< 0.001 compared with the control groups.