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. 2015 Dec 9;5:18007. doi: 10.1038/srep18007

Figure 8. The down-expression of A20 reverses chemokine inhibition induced by IL-1 pre-treatment.

Figure 8

HMC cells, tranfected with the indicated concentration of A20 siRNA (A20si), or with control siRNA (Ctrsi) for 5 h, were cultured for 12 h, followed by the pre-treatment with 5 ng/ml IL-1β for 24 h, and then a treatment with IL-1β for 2 h for A20 protein detection (a), for 1 h for chemokine mRNA detection (b), and for 6 h for chemokine protein detection (c). p-value *< 0.05, compared with non-transfected, IL-1β-pre-treated groups.