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. 2015 Dec 9;5:17998. doi: 10.1038/srep17998

Table 1. Primers for T. harzianum Transformation and Transformants Screening.

PRIMER TARGET REGION SEQUENCE AMPLICON (bp)
Epl1KpnIF Epl-1 Promoter ATGGGTACCGCTGTGGCGATGCTTCTCCAAGT  
Epl1XhoIR Epl-1 Promoter ATGCTCGAGTTTGCCTCCTCCCTTC 950
Epl1HindIIIF Epl-1 Terminator ATGAAGCTTGGTTATTGCATTGCATTGTG  
Epl1bBamHIR Epl-1 Terminator ATGGGATCCTCCTTTGCCATTGCAGAATC 1000
MSEpl1F Epl-1 ORF TTGGCGATGGCTGGATACTACGAT WT: 1090
MSEpl1R Epl-1 ORF TGCCACGTCGATCAGCTTACAAGA epl-1: 3457
HphF Hygromycin ORF GCGGAGGCCATGGATGCGAT  
HphR Hygromycin ORF GCGCTTCTGCGGGCGATTTG 596
RecEpl-1MSF Epl-1 ORF ATGCAATTGTCCAGCCTC  
RecEpl-1MSF Epl-1 ORF CTTGACAGCGACCTGGC 466