Figure 4.

Deletion of Jagged1 in renin cells leads to focal areas of fibrosis and decreased renal function. (A) Immunostaining for α-smooth muscle actin (αSMA) demonstrates focal areas of injured interstitial cells and dilated tubules which express αSMA in mutant animals (age 4 months). (B) Masson’s Trichrome staining demonstrates areas of increased collagen fibers in mutant animals (age 4 months). (C) Blood urea nitrogen and creatinine measured from peripheral blood of control and mutant animals at indicated ages (**P < 0.01; ***P < 0.001, n = 9 and 9 for control animals and n = 6 and 5 for mutant animals at age <6 months and 6–12 months, respectively).