Table 1.
Characteristics of gene-specific real-time qPCR primers
| Gene symbol | Gene name | Accession no.* | Primer sequence | Amplicon size† |
|---|---|---|---|---|
| 18s rRNA | 18s subunit ribosomal RNA | M11188 | (+) acggaccagagcgaaagcat(−) tgtcaatcctgtccgtgtcc | 310 |
| ActB | Beta-actin | NM_031144 | (+) tgtcaccaactgggacgata(−) ggggtgttgaaggtctcaaa | 165 |
| CycA | Cyclophilin A | XM_345810 | (+) agcactggggagaaaggatt(−) agccactcagtcttggcagt | 248 |
| Gapd | Glyceraldehyde-3-phosphate dehydrogenase | NM_017008 | (+) tcaccaccatggagaaggc(−) gctaagcagttggtggtgca | 168 |
| Hmbs | Hydroxymethylbilane synthase | NM_013168 | (+) tcctggctttaccattggag(−) tgaattccaggtgagggaac | 176 |
| Hprt1 | Hypoxanthine guanine phosphoribosyl transferase 1 | NM_012583 | (+) gcagactttgctttccttgg(−) cgagaggtccttttcaccag | 81 |
| Rpl13A | Ribosomal protein L13A | NM_173340 | (+) acaagaaaaagcggatggtg(−) ttccggtaatggatctttgc | 167 |
| Ywhaz | Tyrosine-3-monooxygenase/tryptophan-5-monooxygenase activation protein, zeta | BC094305 | (+) ttgagcagaagacggaaggt(−) gaagcattggggatcaagaa | 136 |
| IL-1β | Interleukin1beta | NM_031512.2 | (+) tgcccgtggagcttccagga(−) tccagctgcagggtgggtgt | 359 |
| IL-6 | Interleukin 6 | NM_012589.2 | (+) tctgtctcgagcccaccaggaa(−) ctggctggaagtctcttgcgga | 91 |
| TNF-α | Tumor necrosis factor-alpha | NM_012675.3 | (+) gaccctcacactcagatcatcttct(−) acgctggctcagccactc | 106 |
| AgRP | Agouti-related protein | NM_033650.1 | (+) ttcccagagttctcaggtctaagt(−) atctagcacctctgccaaagc | 99 |
| NPY | Neuropeptide Y | NM_012614.2 | (+) tggactgaccctcgc(−) tgtctcagggctggatctct | 187 |
| NPYR1 | NPY receptor 1 | NM_001113357.1 | (+) gtctcccgttcacctttgta(−) ccaccagcgatgagaaccaga | 132 |
| NPYR2 | NPY receptor 2 | NM_023968.1 | (+) tgtgcctgccattcactctt(−) gcaacgatgtcggtccaaag | 149 |
| POMC | Proopiomelanocortin | NM_139326.2 | (+) caggacctcaccacggaaag(−) tgacgtacttccggggattt | 114 |
| Pmch | Promelanin-concentrating hormone | NM_012625.1 | (+) cattcaggatggggaaagcc(−) gcctgtgttctttgtggtct | 137 |
| Orexin | Orexin | NM_013179.2 | (+) ttctacaaaggttccctgggc(−) gtagagacggcaggaacacg | 136 |
| LR | Leptin receptor | NM_012596.1 | (+) tgggtttgcgtatggaagtca(−) acgatttcagcagcctctct | 139 |
| FTO | The obesity gene | NM_001039713.1 | (+) gagttctatcagcagtggcag(−) ctcaccaggtcccgaaacaa | 143 |
| Cart | Cocaine-and-amphetamine–regulated transcript | NM_017110.1 | (+) ggcgctgtgttgcagattga(−) agcgtcacacatggggactt | 108 |
*
Genbank accession number of cDNA and corresponding gene, available at http://www.ncbi.nlm.nih.gov/.
†
Amplicon length in base pairs.