Table 1.
Primary antibodies used for immunohistochemistry and Western Analysis
| Host-Antibody | Immunogen-clone, source | Protein MW (kD) | Concentrations used |
|---|---|---|---|
| β-catenin, mouse monoclonal | Synthetic peptide corresponding to residues 27–37 of human β-catenin (Millipore) | ∼97 | 2 μg/mL (WB & IHC) |
| CaSR, mouse monoclonal | Amino acid peptide sequence 15–29 at the extracellular N-terminus of human CaSR (Sigma-Aldrich) | ∼130 | 1–2 μg/mL (WB) 2–4 μg/mL (IHC) |
| CaSR rabbit polyclonal | 20 amino acid peptide sequence near the C-terminus of human CaSR(Millipore) | ∼130 | 2 μg/mL (WB) |
| E-Cadherin, Rabbit monoclonal | Synthetic peptide surrounding residue 780 of human E-Cadherin cytoplasmic region (Cell Signaling) | ∼130 | ∼0.1 μg/mL (WB) 0.2–0.4 μg/mL (IHC) |
| p-120 catenin, mouse monoclonal | C-terminus (Millipore) | 90–120 | ∼1 μg/mL (WB) |
| Rho (-A, -B, -C) clone 55, Mouse monoclonal | Recombinant human RhoA containing amino-acids 1-155. (Millipore) | 22 | 2 μg/mL (WB) |
| β-actin rabbit monoclonal | synthetic peptide corresponding to the N-terminus of human β actin (Licor) | 37 | 0.2 μg/mL (WB |
| β-actin mouse monoclonal | synthetic peptide corresponding to the N-terminus of human β actin (Licor) | 37 | 0.2 μg/mL (WB) |