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. 2015 Nov 24;3(11):e12617. doi: 10.14814/phy2.12617

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Protein content of insulin signaling markers before (basal) and after 7.5 U/kg b.w injection of insulin in visceral adipose tissue (VAT) p-Akt Ser473 and Thr308 (A, B) and subcutaneous adipose tissue (SC) p-Akt Ser473 and Thr308 (C, D). Data are expressed as mean ± standard error, n = 10 per group. Groups which share a letter are not significantly different. Statistical significance is accepted at P < 0.05. In panel B, within +insulin group differences were as follows: OVX ex versus OVX sed P = 0.08, OVX ex versus SHAM sed P = 0.053 as assessed by two-way ANOVA.