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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Integr Biol (Camb). 2015 Nov 13;7(12):1518–1525. doi: 10.1039/c5ib00157a

Figure 3. Metastatic MDA-MB 231 cells grow and invade in the decellularized lung matrix.

Figure 3

(A) IVIS Images showed the growth of MDA-MB 231-Luc cells and MCF7-Luc cells in decellularized lung tissue on Day 6. (B) Quantification of the IVIS results showed the number of MDA-MB 231-Luc cells and MCF7-Luc cells on Day 3, Day 6 and Day 9; n=4. *, p<0.05 (C) Upper panel: Fluorescence images showed the growth of GFP-labeled MDA-MB 231 and MCF7 cells in decellularized lung on Day 9. Bottom panel: IF staining of ColIV (red) and DAPI (Blue) staining on sections of decellularized tissue cultured with MDA-MB 231-Green cells and MCF7-Green cells. Scale bar: 200 μm. (D, E) Bar graphs of the invaded depth (D) and number (E) of MDA-MB 231-Green cells and MCF7-Green cells in the decellularized lung tissue; n=5. **, p<0.01.