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. Author manuscript; available in PMC: 2016 Dec 30.
Published in final edited form as: Integr Biol (Camb). 2015 Nov 13;7(12):1518–1525. doi: 10.1039/c5ib00157a

Figure 5. Knockdown of Zeb1 in MDA-MB 231 cells inhibits colonization of MDA-MB 231 cells in decellularized lung tissue.

Figure 5

(A) Quantitate RT-PCR showed knockdown efficiency of Zeb1 shRNA in MDA-MB 231-Luc cells. (B) Western blot results showed the expression of EMT markers in control and Zeb1-silenced MDA-MB 231-Luc cells. (C) IVIS Images showed the growth of control or Zeb1-silenced MDA-MB 231-Luc cells in decellularized lung tissue on Day 3, Day 6, and Day 9. (D) Quantification of the IVIS images showed the number of control or Zeb1-silenced MDA-MB 231-Luc cells on Day 3, Day 6 and Day 9; n=4. (E) Immunofluorescence staining showed invasion of control or Zeb1-silenced MDA-MB 231-Luc cells in decellularized lung matrix. Blue: DAPI, red: collagen IV. (F) Bar graph showed the invaded depth and number of control or Zeb1-silenced MDA-MB 231-luc cells in the decellularized lung tissue; n=5; **, p<0.01.