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. 2015 Jul 22;1(6):678–694. doi: 10.1016/j.jcmgh.2015.07.006

Figure 10.

Figure 10

Lysosome-associated membrane protein 2 (LAMP-2) maintains acinar cell homeostasis. (A–E). Intrapancreatic content of (A) amylase (immunoblot) and (D) trypsinogen [enterokinase assay (see Materials and Methods)], (B) serum amylase level, and (C) ratio to intrapancreatic amylase, and acinar cell zymogen granules (ZG) number (E) were measured in 6-month-old wild-type and LAMP-2 knockout mice. (E) The average ZG number per acinar cell was quantified on electron micrographs of pancreatic tissue sections. (F–H) Intracellular amylase content (F), and the basal and cholecystokinin (CCK)-induced amylase release (G, H) were measured in pancreatic acini isolated from wild-type and LAMP-2 knockout mice and incubated for 30 minutes with and without CCK-8 at indicated concentrations. (H) Amylase release from wild-type or knockout mice was normalized to that in the absence of CCK. Values are mean ± SEM; n = 3–4 mice of each genotype (B–D), 10–12 cells per group (E), 3–4 acinar cell preparations per group (F–H). *P < .05 versus wild type.