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. 2015 Dec 9;5:17953. doi: 10.1038/srep17953

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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After His-SNAP25 immobilization on 6 independent spots, six samples with the indicated BoNT/E concentrations were injected for 4 sequential 15 min periods (ie. to yield 15, 30, 45 and 60 min cumulated cleavage times). At the end of each 15 min injection, detection of BoNT/E enzymatic activity was achieved by injecting mAb11C3 (n = 3). Each panel represents superimposed sensorgrams obtained for mAb11C3 binding at the indicated times. The last panel illustrates cleavage kinetics at each concentration. The figure is representative of three independent experiments. Note that error bars (SD) are included but too small to be distinguished.