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. 2015 Dec 9;5:17953. doi: 10.1038/srep17953

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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BoNT/A (1 LD₅₀/ml), trypsinized BoNT/E (1 LD₅₀/ml), a mixture of both toxins (1 LD₅₀/ml each) or BoNT-free buffer were injected in parallel for 15 minutes on 4 SNAP-25 spots. BoNT/E activity was revealed using mAb11C3 and anti-mouse Fc antibodies. BoNT/A activity was revealed using mAb10F12 and anti-mouse Fc antibodies. Rapid treatment with pH 2.0 buffer (reg) was used between mAb11C3 and mAb10F12 detection to regenerate the surface. Representative of 3 independent experiments.