Table 1. Measurements of BoNT/E activity by the SPR-based assay.
BoNT/E | Trypsinized BoNT/E | ||||
---|---|---|---|---|---|
Cleavage time | 5 min | 15 min | 5 h | 15 min | 5 h |
LD50/ml | 1 | 1 | 0.01 | 1 | 0.03 |
Detection (RU) | 8.6 ± 1.3* (n = 3) | 16.3 ± 2.7* (n = 5) | 10.4 ± 1.4 (n = 4) | 20.1 ± 5.0 (n = 5) | 4.1 ± 1.3 (n = 3) |
All experiments were performed on a ProteOnXPR36 biosensor except the experiment with 5 min cleavage time that was carried out on a Biacore T200. *Represents mAb11C3 binding without amplification. The LOD values are 1.5 RU for the Biacore T200 versus 1.9 RU (non-amplified) and 0.8 RU (amplified) for the ProteOnXPR36.