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. 2015 Dec 9;5:17953. doi: 10.1038/srep17953

Table 1. Measurements of BoNT/E activity by the SPR-based assay.

  BoNT/E Trypsinized BoNT/E
Cleavage time 5 min 15 min 5 h 15 min 5 h
LD50/ml 1 1 0.01 1 0.03
Detection (RU) 8.6 ± 1.3* (n = 3) 16.3 ± 2.7* (n = 5) 10.4 ± 1.4 (n = 4) 20.1 ± 5.0 (n = 5) 4.1 ± 1.3 (n = 3)

All experiments were performed on a ProteOnXPR36 biosensor except the experiment with 5 min cleavage time that was carried out on a Biacore T200. *Represents mAb11C3 binding without amplification. The LOD values are 1.5 RU for the Biacore T200 versus 1.9 RU (non-amplified) and 0.8 RU (amplified) for the ProteOnXPR36.