Table 3.
Frequencies of CNVs in experimental C. elegans lines at different time intervals of population expansion using single-worm PCR
| Pop ID. generation | Type of rearrangements | Individuals sampled | Number positive for rearrangement | Expectec Hardy-Weinberg frequency | Average copy-number | qPCR | oaCGH |
|---|---|---|---|---|---|---|---|
| 16B.80 | Duplication | 19 | 0 | 0.00 | 1.00 | - | - |
| 16B.140 | 47 | 0 | 0.00 | 1.00 | 0.86 | - | |
| 16B.200 | 43 | 28 | 0.41 | 1.41 | 1.02 | 1.19 | |
| 16E.80 | Duplication | 30 | 1 | 0.02 | 1.02 | 1.08 | - |
| 16E.140 | 30 | 25 | 0.59 | 1.59 | 1.58 | - | |
| 16E.200 | 18 | 18 | 1.00 | 2.00 | 2.39 | 2.08 | |
| 66E.140 | Duplication | 27 | 10 | 0.20 | 1.20 | 1.23 | - |
| 66E.200 | 28 | 22 | 0.54 | 1.54 | 1.67 | 1.33 | |
| 16A.140 | Deletion | 30 | 30 | 1.00 | 0.00 | 0.00 | - |
| 16A.200 | 27 | 27 | 1.00 | 0.00 | 0.00 | 0.05 | |
| 16D.80 | Deletion | 18 | 15 | 0.64 | 0.36 | 0.22 | - |
| 16D.140 | 18 | 28 | 1.00 | 0.00 | 0.000232 | - | |
| 16D.200 | 29 | 29 | 1.00 | 0.00 | 0.000738 | 0.05 | |
| 66B.80 | Deletion | 32 | 0 | 0.00 | 1.00 | 0.73 | - |
| 66B.140 | 15 | 9 | 0.40 | 0.60 | 0.40 | - | |
| 66B.200 | 28 | 28 | 1.00 | 0.00 | 0.0000269 | 0.04 |
Pop ID.Generation refers to the experimental population number and the number of generations of population expansion when sampled. Column 5 displays the frequency of individuals with the rearrangement assuming Hardy-Weinberg equilibrium. Columns 6–8 show the average copy-number per haploid genome for three methods, namely single-worm PCR, qPCR and oaCGH