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. 2015 Dec 9;12:211. doi: 10.1186/s12985-015-0438-6

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Microinjection of dsPns4 inhibited RDV infection and spread in insect vectors in vivo. At 12 days padp, the dissected intestines a and b and salivary glands (c and d) from leafhoppers receiving dsGFP or dsPns4 were immunolabeled with virus-FITC (green) (a and c), Pns4-FITC (green) (b and d) or Pns12-rodanmine (red) (b and d). The images with green fluorescence or red fluorescence were merged under a background of transmitted light. The enlarged images showing green fluorescence (Pns4-FITC) and red fluorescence (Pns12-rodanmine) of the merged images in the boxed areas in each panel, indicated that both of minitubules and diffusion of Pns4 distributed at the edge of viroplasms. fc, filter chamber; amg, anterior midgut; mmg, middle midgut; pmg, posterior midgut; hg, hindgut; mt, Malpighian tubules; sg, salivary gland. Bars, 100 μm (a and c) and 10 μm (b and d)