Table 2.
List of enriched pathways (p <0.05), in which genes predicted to be targeted by differentially expressed miRNAs (P <0.05) between follicular fluid from hyperstimulated vs. unstimulated heifers
| Pathway | miRNAs involved | P value | Top genes |
|---|---|---|---|
| Overexpressed miRNAs (hyp vs. unst) | |||
| TGF-beta signaling pathway | miR-106b, −132, −148b-5p, −182, −212, −374a, −548j | 1.75E-07 | TGFBR2, TGFB2, SMAD2, SMAD3, SMAD4, BMPR2 |
| Axon guidance | miR-22-5p, −30b, −31, −33a-3p, −182, −132, −550a | 2.85E-06 | EFNB1, DCC, EPHB4, EPHA3, PLXNA1, PAK4 |
| MAPK signaling pathway | miR-30b, −106b, −132, −182, −212, −548j, −202-5p | 6.43E-05 | MAP3K1, MAP3K5, KRAS, MRAS, GRB2, FGF7 |
| Endocytosis | miR-33a-3p, −106b, −182, −374a, −374b, −202-5p | 3.83E-04 | RAB11FIP4, RAB11FIP2, EEA1, IGF1R, EPS15, EPN |
| Colorectal cancer | miR-30b, −33a-3p, −106b, −132, −212, −384, −494 | 5.94E-04 | SOS1, FZD3, SMAD2, DCC, MAPK1, BAX |
| Pathways in cancer | miR-33a-3p, −107, −132, −212, −494, −495, −548j | 5.95E-04 | E2F1, FGF18, WNT16, FGF7, PTEN, MITF |
| Wnt signaling pathway | miR-132, −212, −33a-3p, −494, −940, −495, −548j, −107 | 6.73E-04 | LRP5, LRP6, TCF7, PLCB4, DVL3, WNT1, WNT5A |
| Neurotrophin signaling pathway | miR-106b, −30b, −940, −182, −212, −107, | 0.001267 | PIK3R2, NTRK2, NTRK3, RPS6KA6, IRS1, RAC1 |
| Oocyte meiosis | miR-212, −132, −940, 495, −595, −107 | 0.003369 | CDC27, CPEB1, PRKACB, FBXW11, MAPK1, RPS6KA3 |
| GnRH signaling pathway | miR-940, −495 | 0.007085 | PRKCA, SRC, MAP3K2, MAPK14, GRB2, MAP3K3 |
| Underexpressed miRNAs (Hyp vs. Unst) | |||
| Pathways in cancer | miR-659, −141, −190, −449a, −200c, −361-5p, −145 | 9.51E-12 | KRAS, PTEN, VEGFA, STAT5B, MITF, BCL2 |
| MAPK signaling pathway | miR-23a, −23b, −141, −145, −200c, −92a, −125b, −24 | 2.94E-11 | MKNK2, MEF2C, FGF, SOS1, RAS, MAerkPK2 |
| Wnt signaling pathway | miR-125b, −449a, −302e, −145, −23a, −29b-2-5p, −659, −200c | 2.76E-08 | LRP5, LRP6, TCF7, PLCB4, DVL3, WNT1, WNT5A |
| ErbB signaling pathway | miR-23a, −23b, −125b, −206, −302e, −513c | 3.19E-07 | ERBB4, GRB2, SHC1, PIK3R3, PIK3CD, AKT3 |
| Colorectal cancer | miR-659, 23a, −23b, −141, −190, −449a, −200c, −361-5p, −145 | 4.48E-07 | DCC, APPL1, TGFBR2, SMAD3, SMAD4, APC |
| Axon guidance | miR-23a, −23b, −200c, −145 | 7.23E-07 | PLXNC1, EFNB2, PAK4, DCC, SEMA6A, PAK7, MET |
| Neurotrophin signaling pathway | miR-302e, −361-5p, −452, −525-5p, −449a, −659 | 1.82E-06 | NTRK2, NGFR, KRAS, MAP3K1, PIK3R3, PLCG1 |
| Renal cell carcinoma | miR-659, −141, −190, −449a, −200c, −361-5p, −145 | 3.75E-05 | GAB1, MET, SOS1, GRB2, VEGFA, NRAS |
| Focal adhesion | miR-24, −27b, −125a-3p, −139-5p, −206, −200c | 5.04E-05 | ITGB3, PXN, SHC3, ACTB, SRC, PAK2 |
| Regulation of actin cytoskeleton | miR-200c, −145, −27b, −92a, −300, −206 | 8.29E-05 | RAC1, ROCK2, GIT1, PIK3R3, ITGA3, LIMK1 |