Table 5.
Candidate genes mapped in chromosome regions identified as associated with seedling resistance stem rust race TTKSK.
| Durum Wheat Consensus Map (Marone et al., 2012b) | DArT markers in the QTL region based on the map by Marone et al., 2013 | SNP markers in the QTL region based on the map by Maccaferri et al., 2015 | |||
|---|---|---|---|---|---|
| Chr | QTL | Position | Putative function | ||
| 1A-3 | Xgwm633–wPt-7339 | 16.0–24.9 | – | wPt-0432 (14.9 cM from wPt-2847) NBS-LRR | IWA7924 (5.5 cM from wPt-7339) leucine-rich repeat protein kinase putative expressed subfamily LRR-XII |
| 1B | wPt-0308 | 0.0 | Vacuolar sorting protein 39 | – | IWB44529, IWB57219 (2.2 and 2.5 cM from wPt-0308) NBS-LRR |
| wPt-5485 | 81.4 | – | – | IWB19579 (0.6 cM from wPt-5485) CAMK_KIN_like.18—CAMK includes calcium-calmodulin dependent protein kinases | |
| Xgwm124–wPt-7066 | 143.2–148.2 | NBS-LRR (wPt-9032) | wPt-1403 (2.9 cM from Xgwm124) Serine/Threonine protein kinases | IWB50556 (0.8 cM from wPt-7066) NBS-LRR | |
| wPt-4361–wPt-4651 | 206.6–215.6 | – | – | IWB57627, IWB55003 (0.3 and 10.7 cM from wPt-4361) NBS-LRR | |
| 2A-3 | wPt-2858–wPt-9586 | 7.6-8.1 | – | – | IWB38972, IWB51340, IWB70745 (3.8, 4.7, and 4.7 cM from wPt-2858) NBS-LRR |
| 2B | BQ170801 | 10.5 | – | wPt-4026, wPt-8970 (10.0 and 6.8 cM from BQ170801) NBS-LRR | IWB56084, IWB57438 (5.6 and 5.6 cM from BQ170801) leucine-rich repeat protein kinase putative expressed subfamily LRR-XII |
| wPt-1064 | 88.9 | – | wPt-2120b (1.1 cM from wPt-1064) NBS-LRR | IWB36128, IWB31001 (0.0 and 0.3 cM from wPt-1064) CAMK_KIN_like5—CAMK includes calcium-calmodulin dependent protein kinases | |
| wPt-7360 | 172.8 | – | wPt-0189 (3.0 cM from wPt-7360) NBS-LRR | IWA7630 (1.4 cM from wPt-7360) protein kinase family protein putative expressed subfamily RLCK-XII | |
| wPt-4223 | 207.0 | – | Xcdo244 (1.7 cM from wPt-4223) NBS-LRR; wPt-9257 (0.4 cM from wPt-4223) LRR/KIN | IWB21522 (5.4 cM from wPt-4223) protein kinase family protein putative expressed subfamily SD-1c | |
| 3A-2 | Barc45 | 49.5 | – | wPt-2698 (0.3 cM from Barc45) PHD zinc finger protein-like; BJ213673c (0.0 cM from Barc45) CTD-phosphatase-like protein | IWB24651 (6.7 cM from Barc45) protein kinase family protein putative expressed subfamily SD-2b |
| 3B-1 | wPt-5390 | 98.8 | – | wPt-0644 (0.7 cM from wPt-5390) Leucine Rich repeats (2 copies) | IWB47274 (4.9 cM from wPt-5390) protein kinase family protein putative expressed subfamily Raf |
| IWB32491 (2.7 cM from wPt-5390) leucine-rich repeat protein kinase putative expressed subfamily LRR-XIIIa | |||||
| wPt-5055–wPt-4680 | 119.2–128.9 | NBS-LRR (wPt-3729) | wPt-4487a, wPt-0833a, wPt-2951 (0.8, 2.3, and 5.1 cM from wPt-4680) NBS-LRR; wPt-0992a (2.4 cM from wPt-4680) NBS2-RDG2A |
IWB26676, IWB61552 (0.0 and 0.5 cM from wPt-1007) NBS-LRR | |
| 4A | Xgwm937 | 46.3 | – | wPt-6303, wPt-4660 (8.1 and 10.0 cM from Xgwm937) NBS-LRR | – |
| 4B-2 | Xgwm495–wPt-4931 | 78.5–84.0 | – | Xbcd110 (0.8 cM from Xgwm495) filament-like plant protein 4-like | IWB29759 (0.7 cM from wPt-4931) CAMK_KIN_like.11—CAMK includes calcium-calmodulin dependent protein kinases |
| Xgwm1084–wPt-7412 | 106.9–116.9 | – | – | IWB47175 (4.6 cM from Xgwm1084) CAMK_CAMK_like.18—CAMK includes calcium-calmodulin dependent protein kinases | |
| 5A-1 | wPt-7201 | 120.4 | – | – | IWA3827 (3.2 cM from wPt-7201) protein kinase binding |
| 5A-2 | tPt-6495 | 34.6 | – | – | IWB56756 (5.7 cM from tPt-6495) CAMK_CAMK_like_ULKh_APGy.2—CAMK includes calcium-calmodulin depedent protein kinases |
| 5B-1 | wPt-6136*–wPt-9724 | 4.2–16.3 | Protein kinase domain (wPt-6136*) | wPt-9800b (20.7 cM from wPt-9724) NBS-LRR; wPt-0819 (3.8 cM from wPt-9724) LRRs, RI-like subfamily; wPt-8604 (0.8 cM from wPt-9724) LRR receptor-like PK | – |
| Xwmc235 | 175.4 | – | – | IWA6053 (4.8 cM from Xwmc235) CAMK_CAMK_like.1—CAMK includes calcium-calmodulin dependent protein kinases expressed subfamily | |
| 5B-3 | wPt-0054–wPt-0295 | 3.5–5.0 | – | wPt-5168 (5.6 cM from wPt-0054) leucine-rich repeat receptor-like protein kinase | IWB28597 (1.3 cM from wPt-0054) protein kinase family protein putative expressed subfamily Raf |
| 6A | tPt-4209 | 130.0 | – | wPt-8331 (7.8 cM from tPt-4209) NBS-LRR | IWB26292 (0.3 cM from tPt-4209) protein kinase family protein putative expressed subfamily Raf |
| wPt-11612–wPt-6678 | 151.3–156.4 | 1,3-beta-glucan synthase component (wPt-7655) | wPt-3191a (6.3 cM from wPt-11612) NBS-LRR | – | |
| wPt-4229–wPt-8773 | 163.2–177.0 | – | – | IWB22871 (1.6 cM from wPt-4229) protein kinase family protein putative expressed subfamily SD-2b | |
| IWA7572 (7.4 cM from wPt-4229) leucine-rich repeat protein kinase putative expressed subfamily LRR-XII | |||||
| 6B | wPt-9971–wPt-2587 | 62.3–63.8 | – | wPt-7540, wPt-8153 (0.5 and 3.3 cM from wPt-9971) NBS-LRR | IWB48366 (0.4 cM from wPt-9971) NBS-LRR |
| wPt-7846 | 77.5 | F-box domain (wPt-7846) | – | IWB55473 (7.2 cM from wPt-7846) leucine-rich repeat protein kinase putative expressed subfamily LRR-XV | |
| wPt-3606–Xgwm193 | 94.2–107.8 | – | wPt-8721 (2.3 cM from wPt-3606) putative mitogen-activated protein kinase 17-3 | IWB25027 (0.0 cM from Xgwm193) leucine-rich repeat protein kinase putative expressed subfamily LRR-XII | |
| 7A-3 | wPt-0002*–wPt-5092 | 3.9–4.2 | – | – | IWB42938 (5.7 cM from wPt-0002) NBS-LRR |
| wPt-5940 | 137.3 | – | TC92445 (11.1 cM from wPt-5940) pathogenesis-related protein 1-15 | – | |
| 7B | Xwmc606–wPt-7975 | 37.5–46.1 | – | wPt-9800c (12.8 cM from wPt-7975) NBS-LRR | – |
Asterisks (*) indicate the DArT markers projected on the durum wheat consensus map.