(A) Protein:DNA complex formed in the presence of TDP1 and XLF contains TDP1. EMSA was carried out using 100 ng of TDP1 or N-terminally FLAG-tagged TDP1 (FLAG-TDP1), 300 ng of XLF and anti-FLAG monoclonal antibodies (αFLAG) as indicated.
(B) Protein:DNA complex formed in the presence of TDP1 and XLF contains XLF. EMSA was carried out using 100 ng of TDP1, 300 ng of XLF or N-terminally FLAG-tagged XLF (FLAG-XLF) and anti-FLAG monoclonal antibodies (αFLAG) as indicated.