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. Author manuscript; available in PMC: 2015 Dec 9.
Published in final edited form as: Cell Rep. 2013 Aug 22;4(4):649–658. doi: 10.1016/j.celrep.2013.07.029

Figure 1. Asrij Maintains mESC Self-Renewal and Pluripotency.

Figure 1

Analysis of asrij-modulated ESC lines.

(A) Expression of mRNA (i) and protein (ii).

(B) Morphology.

(C) Quantitative analysis of proliferation over 48 hr (i), and population doubling time analysis (i and ii). Panel (ii) shows increased doubling time for +/− cells and faster doubling for OV cells, and panel (iii) shows reduced and increased percentages of dividing cells for +/− and OV cells, respectively.

(D) Cell-cycle profiles analyzed by flow cytometry. Bars represent % distribution of cells in G1, S, and G2-M.

(E) Image (i) and graph (ii) showing AP+ colonies in a clonal assay.

(F) Quantitative RT-PCR (qRT-PCR) analysis for pluripotency markers.

Statistical significance is indicated by **p < 0.05, ***p < 0.001. Error bars show SD of the mean. Scale bar, 100 μM. See also Figure S1.