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. Author manuscript; available in PMC: 2015 Dec 9.
Published in final edited form as: Biochem J. 2013 Jan 15;449(2):491–496. doi: 10.1042/BJ20121155

Table 1. Steady-state kinetic parameters for KDM4E determined under different experimental conditions.

kcat (s−1) KM (μM)
Assay H3K9me3 H3K9me2 H3K9me3 H3K9me2
MALDI-MS (5°C) 0.048 ± 0.001 0.040 ± 0.001 14.0 ± 1.9 18.2 ± 3
NMR (25 °C) 0.018 ± 0.002 0.02 ± 0.001 203 ± 79 282 ± 36
HCHO release (37°C) 0.076 ± 0.01 0.065 ± 0.002 23 ± 4 25 ± 3

Substrates were 8mer peptides. Mean values and standard deviations for kcat and KM (n = 3) are shown.