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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: J Neuroimmunol. 2015 Jun 12;285:137–142. doi: 10.1016/j.jneuroim.2015.06.003

Figure 3.

Figure 3

Peripheral inflammation triggered by PIC challenge increases hippocampal production of complement factor B (CfB). Mice were i.p. injected with 12 mg/kg of PIC or saline. After 24 h, the brains were perfusion fixed and sectioned (35 μm). Sections were stained with specific antibodies and imaged by confocal microscopy. Upper panel; Composites of 10X images of hippocampi from saline and PIC-challenged mice stained with anti-CfB antibody. Lower panel; Cellular localization of CfB in the CA3 region of the hippocampus. Cerebral sections were double stained with anti-CfB and anti-NeuN (neuronal marker), anti-GFAP (astrocytic marker) or anti-Iba1 (microglial marker) antibodies and imaged at 20X.