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. 2015 Dec 9;2:15046. doi: 10.1038/mtm.2015.46

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Reprogramming XCGD PB CD34+ cells into iPSCs. (a) Schematic overview of the reprogramming process using a Sendai virus (SeV) vector carrying the four Yamanaka factors to transduce peripheral blood (PB) CD34⁺ cells. XCGD iPSC (#8) was subjected to detailed characterization and used for subsequent experiments. Embryoid body medium (EBM). (b) XCGD iPSCs stained positive for ALP, SSEA-4, Tra-1–60, and Tra-1–81. (c) XCGD iPSCs could form teratomas consisting of tissue derived from the three germ layers endoderm, mesoderm, and ectoderm. (d) A normal karyotype was exhibited. (e) Confirmation of the point mutation CYBB: c.1448G>A (p.Trp483X). Bars = 200 μm.