Figure 4.

Identification of neutrophil populations expressing ectopic gp91phox with ROS producing capacity. EFS and SFFV represents neutrophils differentiated from iPSCs where gp91phox expression is driven by these two promoters specifically. Control cells were those differentiated from untransduced healthy or XGCD-iPSCs. (a) Expression of gp91phox. Top row indicates expression in mature (CD64^highCD15^high) cells. Bottom row indicates expression in developing (CD64^dullCD15^dull) neutrophils. In this population, gp91phox expression could only be detected in the transduced groups. Gray indicates the isotype control and black line indicates gp91phox expression. (b) Generation of ROS in mature (top row) and developing (bottom row) neutrophils. Nonphysiological ROS production was only detected in the transduced groups where cells were ectopically expressing gp91phox. Gray indicates no stimulation and black line indicates stimulation with phorbol myristate acetate. All numbers indicate the MFI.