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. 2015 Dec 9;2:15046. doi: 10.1038/mtm.2015.46

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Developing neutrophils become mature neutrophils with an extended differentiation period. After 3 days of differentiation, a combination of both developing (CD64^dullCD15^dull) and mature (CD64^highCD15^high) neutrophilss could be seen in the culture at this point (first column; Pre-sorting). The developing fraction was sorted by FACS and the purity of this sorted fraction was checked (second column; Purity). After two further days of differentiation, the appearance of mature cells that originated from the sorted developing fraction could be identified (third column; Differentiated).