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Alcian blue staining of human dermal fibroblasts encapsulated in alginate after 8 days in culture for (a) the control group in the absence of growth factors, (b) the treated group in the absence of growth factors, (c) the control group in the presence of growth factors, (d) the treated group in the presence of growth factors and (e) the alginate disc only. A blue dark region surrounding the cells shows the presence of GaG (arrow). All micrographs are at the same magnification.
