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. 2015 Dec 9;10(12):e0144776. doi: 10.1371/journal.pone.0144776

Fig 3. Induction of DEFB131 gene expression by LTA is p38MAPK- and NF-κB-dependent.

Fig 3

(A) Phosphorylation of p38MAPK and IκBα determined by western blot after LTA stimulation. LTA stimulated an increase in p38MAPK and IκBα phosphorylation in RWPE-1 cells. In contrast, expression of IκBα was significantly reduced in a time-dependent manner. (B) Inhibition of p38MAPK and NF-κB blocks DEFB131 production. ELISA analysis showed that SB203580 (p38MAPK inhibitor) and Bay11-7082 (NF-κB inhibitor) inhibited secretion of LTA-induced DEFB131 protein. Asterisks (* and **) represent statistical significance at P < 0.0001 and P < 0.002. (C) Immunocytochemistry of DEFB131 after SB203580 or Bay11-7082 treatment showed a reduction in DEFB131 protein expression.