Fig 5. DEFB131 induces cytokine and chemokine expression in RWPE-1 cells.
(A) DEFB131 mRNA upregulation. RWPE-1 cells were transfected with an empty vector or the DEFB131-DDK-Myc vector. Quantitative real-time PCR analysis was used to determine mRNA levels. Expression levels were calculated from CT values and normalized to ACTB. Asterisks represent statistical significance at P < 0.0001. (B) Upregulation of DEFB131 protein. DEFB131 protein levels were analyzed by western blot in cell lysates. (C) Overexpression of secreted DEFB131 protein by western blot in culture supernatants. (D, E) Gene expression profiles for cytokines and chemokines in DEFB131-activated RWPE-1 cells were determined using quantitative real-time PCR analysis. Asterisks (* and **) represent statistical significance at P < 0.01 and P < 0.05.