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. 2015 Dec 9;10(12):e0144776. doi: 10.1371/journal.pone.0144776

Table 1. Primer sequences and RT-PCR conditions.

Gene Accession numbers Primer sequence Annealing temperature Product size
DEFB131 NM_001040448 For–TCCAGCCAGAAGCTTCATTT 65°C 158 bp
NM_001040448 Rev–ACCACTTCTTTTGTCCGTCA 65°C 158 bp
TLR2 NM_003264 For–GATGCCTACTGGGTGGAGAA 67°C 393 bp
NM_003264 Rev–CGCAGCTCTCAGATTTACCC 67°C 393 bp
TLR6 NM_006068 For–AGACCTACCGCTGAAAACCAA 62°C 225 bp
NM_006068 Rev–ACTCACAATAGGATGGCAGGA 62°C 225 bp
CD14 NM_000591 For–CGTGGGCGACAGGGCGT 67°C 777 bp
NM_000591 Rev–TAAAGGTGGGGCAAAGGGTT 67°C 777 bp
ACTB NM_001101 For–CCATCGAGCACGCATCGTC 68°C 375 bp
NM_001101 Rev–CTCGGTGAGGATCTTCATGA 68°C 375 bp

For, forward; Rev, reverse