Table 1. Primer sequences and RT-PCR conditions.
Gene | Accession numbers | Primer sequence | Annealing temperature | Product size |
---|---|---|---|---|
DEFB131 | NM_001040448 | For–TCCAGCCAGAAGCTTCATTT | 65°C | 158 bp |
NM_001040448 | Rev–ACCACTTCTTTTGTCCGTCA | 65°C | 158 bp | |
TLR2 | NM_003264 | For–GATGCCTACTGGGTGGAGAA | 67°C | 393 bp |
NM_003264 | Rev–CGCAGCTCTCAGATTTACCC | 67°C | 393 bp | |
TLR6 | NM_006068 | For–AGACCTACCGCTGAAAACCAA | 62°C | 225 bp |
NM_006068 | Rev–ACTCACAATAGGATGGCAGGA | 62°C | 225 bp | |
CD14 | NM_000591 | For–CGTGGGCGACAGGGCGT | 67°C | 777 bp |
NM_000591 | Rev–TAAAGGTGGGGCAAAGGGTT | 67°C | 777 bp | |
ACTB | NM_001101 | For–CCATCGAGCACGCATCGTC | 68°C | 375 bp |
NM_001101 | Rev–CTCGGTGAGGATCTTCATGA | 68°C | 375 bp |
For, forward; Rev, reverse