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. Author manuscript; available in PMC: 2016 Oct 24.
Published in final edited form as: FEBS Lett. 2015 Oct 3;589(21):3237–3241. doi: 10.1016/j.febslet.2015.09.020

Table 1.

Deamidation rate for side chain NH2 groups in Scr

Residue Rate (day−1) t1/2 (day) Sequence Predicted t1/2 (day)
Asn −5 (2.24 ± 1.44) × 10−3 310 ± 200 VNA 27.5
Asn −3 (2.68 ± 0.14) × 10−2 26 ± 1 ANG 1.05
Gln 4 (6.80 ± 1.20) × 10−3 102 ± 18 RQR 2300
Gln 12 (1.44 ± 0.83) × 10−3 483 ± 279 YQT 5600

The deamidation rate is calculated for the sum of the intensities of the two side chain NH2 peaks for each residue in Fig. 4a. The predicted t1/2 for the flanking tripeptide sequence (in single letter amino acid code) is derived from Robinson et al. [4; 5]. Fitted deamidation rates for Asn 23, Gln 44, Gln 50, and Asn 51 are < 1.25 × 10−3day−1 and are not significantly different from zero (not shown).