(A) Antiproliferative activity of Cmpd-A in multiple cancer cell lines. DU145, COLO205, NIH-OVCAR3, RKO, ES2, SK-OV3, PC-3, SW620, and CAPAN-2 cell lines were treated with Cmpd-A for 3 days at the indicated concentrations. The relative ATP concentration was calculated based on the chemiluminescence compared with the 0 nM chemiluminescence value (control). Data are presented as mean ± standard deviation (n = 3). (B) Correlation between the antiproliferative activity of Cmpd-A and CENP-E mRNA expression in cancer cell lines. The X and Y axes indicate the relative ATP level at 300 nM Cmpd-A treatment and CENP-E mRNA levels in the 14 indicated cancer cell lines, respectively. The relative ATP concentration was calculated based on chemiluminescence compared with the 0 nM chemiluminescence value (control) in each cell line. The raw data of CENP-E mRNA expression was downloaded from the Cancer Cell Line Encyclopedia (http://www.broadinstitute.org/ccle/data/browseData) and processed with MAS 5.0 algorithm.