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. 2015 Dec 9;10(12):e0144254. doi: 10.1371/journal.pone.0144254

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© 2015 Kurita et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) The pair of pin electrodes for single well of 96multi-well plate. (B) Merge image between bright-field (BF) and fluorescence images of HEK cells 1, 4, 7, and 12 days after W/O droplet electroporation. All scale bars, 30 μm. (C) HEK293 cells transfected with Venus fluorescent plasmid 1, 6, and 9 days after W/O droplet electroporation. Transfection efficiency was compared with 5-minute application time. (D) HEK293 cells transfected with Venus plasmid with application times of 1, 5, and 10 minutes 6 and 9 days after electroporation.