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. 2015 Dec 9;10(12):e0144254. doi: 10.1371/journal.pone.0144254

Fig 3. Fibroblast, SCN neural cells and HEK293 cells transfected Venus plasmid by W/O droplet electroporation.

Fig 3

(A) Bright field, fluorescence and merge images of fibroblast cells from a subject aged 81 years old one day after electroporation. (B) 7 days after W/O droplet electroporation. (C) Bright field, fluorescence and merge images of SCN neural cells three days after W/O droplet electroporation. SCN cells had been differentiated to neural cells by incubation at higher culture temperature [26]. (D) Twelve days after W/O droplet electroporation. All scale bars (A-D), 30 μm. (E) Bright field, fluorescence and merge images of HEK293 cells colonies were picked up and cultured for one month after W/O droplet electroporation. (F) Bright field, fluorescence and merge images of HEK293 cells colonies were cultured though frozen stock for a total of more than two months after W/O droplet electroporation. scale bars (D-E), 100 μm.